Latvians in Michigan (Discovering the Peoples of Michigan)


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With a myriad of imaging options, how do you know which method is the most appropriate for your needs? The purpose of this blog is to bring clarity to the use of several preclinical imaging modalities available at MI Bioresearch. Images are generally desired to be used as aesthetically appealing data; however, the real magic is within the pixels that make up an image.

Each pixel contains a value which relates to the brightness of that pixel. These numerical values may be used to quantify the images, thus providing data far more informative than a pretty picture. Tumor burden quantification is one of the most common analyses performed. When deciding which imaging modality is best suited to a study, some considerations need to be reviewed, such as, if the model of interest is luciferase enabled or if the model is disseminated or focal.

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BLI is typically suggested if the model of interest is luciferase enabled and is disseminated. In this setting, BLI is an ideal methodology to quantitatively understand the in vivo efficacy of a therapeutic test agent. MRI is our second most utilized modality and delivers a stack of 2D images which are used to calculate tumor volume. Unlike BLI, MRI provides a familiar measurement, mm 3 , which is highly accurate and does not require the tumor cells to be modified to express luciferase. In addition, MRI provides an accurate indication of tumor location.

MR imaging is typically used in orthotopic models where calipers cannot be used. CT is useful for analyzing lung tumor models due to its high resolution and strong tissue contrast against air. This is also the clinical standard for examining lung tissue.

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The output is a stack of 2D images and allows for tumor volume mm 3 determination. This is the only modality that uses radiation x-rays for tumor burden quantification. Biodistribution is another common analysis performed at MI Bioresearch. Biodistribution determines test material distribution within a subject over time. MRI and CT can also be used for biodistribution but this application is not as common. PET imaging is the preferred modality for biodistribution due to its great sensitivity, accuracy of quantification, and clinical translation.

Results can be delivered as percent injected dose or percent injected dose per gram over time.

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    Latvians in Michigan (Discovering the Peoples of Michigan) Latvians in Michigan (Discovering the Peoples of Michigan)
    Latvians in Michigan (Discovering the Peoples of Michigan) Latvians in Michigan (Discovering the Peoples of Michigan)
    Latvians in Michigan (Discovering the Peoples of Michigan) Latvians in Michigan (Discovering the Peoples of Michigan)
    Latvians in Michigan (Discovering the Peoples of Michigan) Latvians in Michigan (Discovering the Peoples of Michigan)
    Latvians in Michigan (Discovering the Peoples of Michigan) Latvians in Michigan (Discovering the Peoples of Michigan)

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